Marko Usaj

Research engineer

Department of Chemistry and Biomedical Sciences Faculty of Health and Life Sciences

marko.usaj@lnu.se

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Publications

Article in journal (Refereed)

Berg, A., Velayuthan, L.P., Månsson, A., Usaj, M. (2024). Cost-Efficient Expression of Human Cardiac Myosin Heavy Chain in C2C12 Cells with a Non-Viral Transfection Reagent. International Journal of Molecular Sciences. 25 (12).
Status: Published
Berg, A., Velayuthan, L.P., Tågerud, S., Ušaj, M., Månsson, A. (2024). Probing actin-activated ATP turnover kinetics of human cardiac myosin II by single molecule fluorescence. Cytoskeleton.
Status: Epub ahead of print
Usaj, M., Pavlin, M., Kanduser, M. (2024). Feasibility Study for the Use of Gene Electrotransfer and Cell Electrofusion as a Single-Step Technique for the Generation of Activated Cancer Cell Vaccines. Journal of Membrane Biology.
Status: Epub ahead of print
Velayuthan, L.P., Moretto, L., Tågerud, S., Ušaj, M., Månsson, A. (2023). Virus-free transfection, transient expression, and purification of human cardiac myosin in mammalian muscle cells for biochemical and biophysical assays. Scientific Reports. 13 (1).
Status: Published
Salhotra, A., Rahman, M.A., Ruijgrok, P.V., Meinecke, C.R., Ušaj, M., et al. (2023). Exploitation of Engineered Light-Switchable Myosin XI for Nanotechnological Applications. ACS Nano. 17 (17). 17233-17244.
Status: Published
Månsson, A., Ušaj, M., Moretto, L., Matusovsky, O., Velayuthan, L.P., et al. (2023). New paradigms in actomyosin energy transduction : Critical evaluation of non-traditional models for orthophosphate release. Bioessays. 45 (9).
Status: Published
Ušaj, M., Moretto, L., Månsson, A. (2022). Critical Evaluation of Current Hypotheses for the Pathogenesis of Hypertrophic Cardiomyopathy. International Journal of Molecular Sciences. 23 (4).
Status: Published
Moretto, L., Ušaj, M., Matusovsky, O., Rassier, D.E., Friedman, R., et al. (2022). Multistep orthophosphate release tunes actomyosin energy transduction. Nature Communications. 13 (1).
Status: Published
Salhotra, A., Zhu, J., Surendiran, P., Meinecke, C.R., Lyttleton, R., et al. (2021). Prolonged function and optimization of actomyosin motility for up scaled network-based biocomputation. New Journal of Physics. 23.
Status: Published
Vemula, V., Huber, T., Ušaj, M., Bugyi, B., Månsson, A. (2021). Myosin and gelsolin cooperate in actin filament severing and actomyosin motor activity. Journal of Biological Chemistry. 296.
Status: Published
Ušaj, M., Moretto, L., Vemula, V., Salhotra, A., Månsson, A. (2021). Single molecule turnover of fluorescent ATP by myosin and actomyosin unveil elusive enzymatic mechanisms. Communications Biology. 4 (1). 1-12.
Status: Published
Kandušer, M., Kokalj Imsirovic, M., Ušaj, M. (2019). The Effect of Lipid Antioxidant α-Tocopherol on Cell Viability and Electrofusion Yield of B16-F1 Cells In Vitro. The Journal of Membrane Biology. 252 (1). 105-114.
Status: Published
Rahman, M.A., Ušaj, M., Rassier, D.E., Månsson, A. (2018). Blebbistatin Effects Expose Hidden Secrets in the Force-Generating Cycle of Actin and Myosin. Biophysical Journal. 115 (2). 386-397.
Status: Published
Ušaj, M., Zattelman, L., Regev, R., Shneyer, B.I., Wiesel-Motiuk, N., et al. (2018). Overexpression and purification of human myosins from transiently and stably transfected suspension adapted HEK293SF-3F6 cells. Analytical Biochemistry. 558 (1). 19-27.
Status: Published
Ušaj, M., Henn, A. (2017). Kinetic adaptation of human Myo19 for active mitochondrial transport to growing filopodia tips. Scientific Reports. 7 (1).
Status: Published
Zattelman, L., Regev, R., Ušaj, M., Reinke, P.Y.A., Giese, S., et al. (2017). N-terminal splicing extensions of the human MYO1C gene fine-tune the kinetics of the three full-length myosin IC isoforms. Journal of Biological Chemistry. 292 (43). 17804-17818.
Status: Published
Shneyer, B.I., Ušaj, M., Wiesel-Motiuk, N., Regev, R., Henn, A. (2017). ROS induced distribution of mitochondria to filopodia by Myo19 depends on a class specific tryptophan in the motor domain. Scientific Reports. 7 (1).
Status: Published
Shneyer, B.I., Ušaj, M., Henn, A. (2016). Myo19 is an outer mitochondrial membrane motor and effector of starvation-induced filopodia. Journal of Cell Science. 129 (3). 543-556.
Status: Published
Zoabi, M., Nadar-Ponniah, P.T., Khoury-Haddad, H., Ušaj, M., Budowski-Tal, I., et al. (2014). RNA-dependent chromatin localization of KDM4D lysine demethylase promotes H3K9me3 demethylation. Nucleic Acids Research. 42 (21). 13026-13038.
Status: Published
Kandušer, M., Ušaj, M. (2014). Cell electrofusion: past and future perspectives for antibody production and cancer cell vaccines. Expert Opinion on Drug Delivery. 11 (12). 1885-1898.
Status: Published
Rems, L., Ušaj, M., Kandušer, M., Reberšek, M., Miklavčič, D., et al. (2013). Cell electrofusion using nanosecond electric pulses. Scientific Reports. 3.
Status: Published
Ušaj, M., Flisar, K., Miklavcic, D., Kanduser, M. (2013). Electrofusion of B16-F1 and CHO cells: the comparison of the pulse first and contact first protocols. Bioelectrochemistry. 89. 34-41.
Status: Published

Conference paper (Refereed)

Månsson, A., Ušaj, M. (2018). Challenges in TIRF-Microscopy Based Single Molecule ATPase and Binding Assays for Myosin and Actin. Biophysical Journal Supplement 1. 211a-211a.
Henn, A., Shneyer, B., Ušaj, M. (2016). Myosin 19 is an Outer Mitochondrial Membrane Motor and Effector of Starvation Induced Filopodia with Unique Kinetic Features. Biophysical Journal Supplement 1. 615a-616a.
Shneyer, B., Ušaj, M., Henn, A. (2015). Myosin 19 is Anchored to the Mitochondria, Affecting its Localization and Morphology. Biophysical Journal supplement 1. 303a.

Chapter in book (Refereed)

Ušaj, M., Kandušer, M. (2015). Modified Adherence Method (MAM) for Electrofusion of Anchorage-Dependent Cells. Cell Fusion : Overviews and Methods. New York, NY, Humana Press. 203-216.

Article, review/survey (Refereed)

Månsson, A., Ušaj, M., Moretto, L., Rassier, D.E. (2018). Do Actomyosin Single-Molecule Mechanics Data Predict Mechanics of Contracting Muscle?. International Journal of Molecular Sciences. MDPI. 19 (7).
Status: Published